Affymetrix Arrays
The centre has the latest Affymetrix processing and scanning hardware enabling all current Affymetrix array types to be run. The Affymetrix system is currently operated as fee-for-service only.
 
Gene Expression
Affymetrix microarrays are available in different formats for gene expression studies. The traditional genome expression arrays contain probes which target the 3' end of transcripts and are available for the largest range organisms including many species of agricultural importance. These arrays are ideally performed with a mininimum of 2ug of starting RNA (using the standard single cycle labelling method).
Exon and Genearrays are newer microarrays which have probes targetting whole transcripts (not just the 3' end) and are available for expression studies in Human, Mouse and Rat species. Exon arrays enable the profiling of alternative splicing events in these species on a single array. Genearrays contain a subset of probes selected from the Exon arrays and have the advantage of requiring only 150 ng of input RNA. For more detail on choosing an appropriate Affymetrix array for studying gene expression please contact the centre or visit the Affymetrix website.
NB. RNA samples submitted for Affymetrix arrays are initially assessed for quality using an Agilent Bioanalyzer.
Complete this order form to purchase Affymetrix Expression Arrays.
Gene Regulation
Promoter and high density Tiling arrays are available for applications including identifying DNA methylation sites and transcription factor binding sites (ChIP-chip assay). For more detail on Gene Regulation arrays please contact the centre or visit the Affymetrix website.
Genotyping
The latest Genechip SNP 6.0 arrays contain approximately 1.8 million probes and can be used for many different genotyping applications. Approximately half the probes on the SNP 6.0 arrays are SNP targetting and the remainder are non-polymorphic for Copy Number detection.
More focused genotyping can be performed using the Affymetrix Targetted Genotyping (TG) array system. TG experiments are performed using Universal Tag arrays in combination with a specific MIP probe labelling mixture. Users can design assays for a custom set of SNP regions or choose from the range of ready-made Affymetrix assays. The centre's GSC3000 7G scanner is able to scan the 4 fluorphores used in this application. For more detail on Genotyping arrays please contact the centre or visit the Affymetrix website.
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